发明申请
US20120196326A1 Mammalian Cell Lines for Increasing Longevity and Protein Yield from a Cell Culture
有权
基本信息:
- 专利标题： Mammalian Cell Lines for Increasing Longevity and Protein Yield from a Cell Culture
- 专利标题（中）：哺乳动物细胞系从细胞培养增加寿命和蛋白质产量
- 申请号：US13413871 申请日：2012-03-07
- 公开(公告)号：US20120196326A1 公开(公告)日：2012-08-02
- 发明人： David M. Goldenberg , Zhengxing Qu , Chien Hsing Chang , Edmund A. Rossi , Jeng-Dar Yang , Diane Nordstrom
- 申请人： David M. Goldenberg , Zhengxing Qu , Chien Hsing Chang , Edmund A. Rossi , Jeng-Dar Yang , Diane Nordstrom
- 申请人地址： US NJ Morris Plains
- 专利权人： IMMUNOMEDICS, INC.
- 当前专利权人： IMMUNOMEDICS, INC.
- 当前专利权人地址： US NJ Morris Plains
- 主分类号： C12P21/00
- IPC分类号： C12P21/00 ; C12N9/00
摘要：
Disclosed are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. Cells transfected with an apoptosis-inhibiting gene or vector, such as a triple mutant Bcl-2 gene, can survive longer in culture, resulting in extension of the state and yield of protein biosynthesis. Such transfected cells exhibit maximal cell densities that equal or exceed the maximal density achieved by the parent cell lines. Transfected cells can also be pre-adapted for growth in serum-free medium, greatly decreasing the time required to obtain protein production in serum-free medium. In certain methods, the pre-adapted cells can be used for protein production following transformation under serum-free conditions. The method preferably involves eukaryotic cells, more preferably mammalian cells.
摘要（中）：
公开了用于增加细胞培养物的寿命并允许增加蛋白质生产的组合物和方法，优选重组蛋白，例如抗体，肽，酶，生长因子，白细胞介素，干扰素，激素和疫苗。 用凋亡抑制基因或载体如三重突变体Bcl-2基因转染的细胞可以在培养物中更长时间地存活，导致蛋白质生物合成的状态和产量的延长。 这样的转染细胞表现出最大的细胞密度，其等于或超过由亲本细胞系实现的最大密度。 转染的细胞也可以预先适应于无血清培养基中生长，大大减少了在无血清培养基中获得蛋白质产生所需的时间。 在某些方法中，预先调节的细胞可以在无血清条件下转化后用于蛋白质生产。 该方法优选涉及真核细胞，更优选哺乳动物细胞。
公开/授权文献:
IPC结构图谱:
| C | 化学；冶金 |
| --C12 | 生物化学；啤酒；烈性酒；果汁酒；醋；微生物学；酶学；突变或遗传工程 |
| ----C12P | 发酵或使用酶的方法合成目标化合物或组合物或从外消旋混合物中分离旋光异构体 |
| ------C12P21/00 | 肽或蛋白质的制备 |