发明申请
US20110183405A1 MODIFIED MULTIWELL PLATE FOR BIOCHEMICAL ANALYSES AND CELL CULTURE EXPERIMENTS.
审中-公开
基本信息:
- 专利标题： MODIFIED MULTIWELL PLATE FOR BIOCHEMICAL ANALYSES AND CELL CULTURE EXPERIMENTS.
- 专利标题（中）：用于生物化学分析和细胞培养实验的改性多孔板。
- 申请号：US12809211 申请日：2008-12-16
- 公开(公告)号：US20110183405A1 公开(公告)日：2011-07-28
- 发明人： Tilo Pompe , Kristina Lehmann , Mirko Nitschke , Carsten Werner
- 申请人： Tilo Pompe , Kristina Lehmann , Mirko Nitschke , Carsten Werner
- 优先权： DE102007055865.3 20071219
- 国际申请： PCT/EP08/67647 WO 20081216
- 主分类号： C12M1/00
- IPC分类号： C12M1/00
摘要：
The invention relates to a modified multi-well plate for biochemical analyses and cell culture experiments, which can be obtained through a method for functionalization. The method comprises the following process steps: a) Treatment of the multi-well plate by means of an ammonia low-pressure plasma, so that reactive amino groups are formed on the plate surface; b) Application of an aqueous or alcoholic solution of a maleic anhydride copolymer; c) Drying the solution of the maleic anhydride copolymer on the surface; d) Heat treatment for the covalent bonding of the maleic anhydride copolymer to the multi-well plate; e) Rinsing with aqueous solution to eliminate unbound and water-soluble maleic anhydride copolymer f) Heat treatment to reestablish the reactivity of the anhydride groups on the maleic anhydride copolymer.
摘要（中）：
本发明涉及用于生物化学分析和细胞培养实验的改良多孔板，其可以通过官能化方法获得。 该方法包括以下工艺步骤：a）通过氨低压等离子体处理多孔板，从而在板表面上形成反应性氨基; b）马来酸酐共聚物的水溶液或醇溶液的应用; c）将马来酸酐共聚物的溶液干燥在表面上; d）马来酸酐共聚物与多孔板的共价键合的热处理; e）用水溶液冲洗以消除未结合和水溶性马来酸酐共聚物f）热处理以重新建立马来酸酐共聚物上酸酐基团的反应性。