发明申请
US20080131871A1 Compositions and methods for identifying antigens which elicit an immune response
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基本信息:
- 专利标题： Compositions and methods for identifying antigens which elicit an immune response
- 专利标题（中）：用于鉴定引发免疫应答的抗原的组合物和方法
- 申请号：US11896337 申请日：2007-08-31
- 公开(公告)号：US20080131871A1 公开(公告)日：2008-06-05
- 发明人： Si-Yi Chen , Zhaoyang You
- 申请人： Si-Yi Chen , Zhaoyang You
- 申请人地址： US NC Winston-Salem
- 专利权人： Wake Forest University Health Sciences
- 当前专利权人： Wake Forest University Health Sciences
- 当前专利权人地址： US NC Winston-Salem
- 主分类号： C12Q1/70
- IPC分类号： C12Q1/70 ; C12Q1/68
摘要：
This invention relates to an expression vector wherein said expression vector comprises a polynucleotide promoter sequence, a polynucleotide encoding a signal sequence, a polynucleotide encoding an antigen protein or peptide, a polynucleotide encoding a cell binding element, and a polynucleotide polyadenylation sequence all operatively linked. More particularly, it relates to the method of eliciting an immune response directed against an antigen in a mammal comprising the steps of introducing the expression vector into a cell, expressing the vector to produce an antigen under conditions wherein the antigen is secreted from the cell, endocytosing the secreted antigen into the cell, processing the antigen, and presenting fragments to a receptor to elicit a T-cell response. In addition, this invention relates to a vaccine and a method of use. The invention also relates to the method of identifying MHC-II restricted epitopes.
摘要（中）：
本发明涉及表达载体，其中所述表达载体包含多核苷酸启动子序列，编码信号序列的多核苷酸，编码抗原蛋白或肽的多核苷酸，编码细胞结合元件的多核苷酸和全部可操作地连接的多核苷酸聚腺苷酸化序列。 更具体地说，本发明涉及在哺乳动物中引发针对抗原的免疫应答的方法，包括以下步骤：将表达载体导入细胞，在其中抗原从细胞分泌的条件下表达载体产生抗原， 将分泌的抗原内吞到细胞中，处理抗原，并将片段呈递给受体以引发T细胞应答。 此外，本发明涉及疫苗和使用方法。 本发明还涉及鉴定MHC-II限制性表位的方法。