基本信息:
- 专利标题： ＨｐＧＡＳ1 유전자 파쇄 효모 균주 및 이를 이용한 재조합 단백질의 생산 방법
- 专利标题（英）：Hpgas1 gene deleted yeasts and methods of producing recombinant proteins using them
- 专利标题（中）：HPGAS1基因的删除和使用它们生产重组蛋白的方法
- 申请号：KR1020130045467 申请日：2013-04-24
- 公开(公告)号：KR1020130142900A 公开(公告)日：2013-12-30
- 发明人： 권오석 , 황동현 , 오두병
- 申请人： 한국생명공학연구원
- 申请人地址： ***, Gwahak-ro, Yuseong-gu, Daejeon, (***-***), Republic of Korea
- 专利权人： 한국생명공학연구원
- 当前专利权人： 한국생명공학연구원
- 当前专利权人地址： ***, Gwahak-ro, Yuseong-gu, Daejeon, (***-***), Republic of Korea
- 代理人： 이원희
- 优先权： KR1020120065686 2012-06-19
- 主分类号： C12N1/19
- IPC分类号： C12N1/19 ; C12N15/81
摘要：
The present invention relates to a method for using mutated strain, in which protein secretion ability is enhanced by damaging novel genes, for producing heterologous recombinant proteins. More specifically, the present invention searches Hansenula polymorpha genome sequencing and obtains HpGAS gene candidate groups which code Gas1 protein having beta-1,3-glucanosyltransglycosylase activities and checks novel genes (HpGAS1) among the same and deletes corresponding genes and verifies the functions so that form of cells can be changed, sensitivity of a cell wall synthesis inhibitor can be increased, total amount of protein secretion can be increased, secretion amount of the recombinant protein can be drastically increased, and HpGAS1 gene deleted yeast can be used as a resource for secretory expression, purification, and harvest of heterologous recombinant proteins.
摘要（中）：
本发明涉及一种使用突变菌株的方法，其中蛋白质分泌能力通过损害新基因而增强，用于产生异源重组蛋白。 更具体地，本发明搜索多形汉逊酵母基因组测序，并获得编码具有β-1,3-葡聚糖糖基转移酶活性的Gas1蛋白质的HpGAS基因候选基因并检查相同的新基因（HpGAS1），并删除相应的基因并验证功能，使得 可以改变细胞形态，增加细胞壁合成抑制剂的敏感性，可以增加蛋白质分泌的总量，重组蛋白的分泌量可以大大提高，HpGAS1基因缺失的酵母可以用作 分泌表达，纯化和异源重组蛋白的收获。
公开/授权文献:
- KR101498012B1 ＨｐＧＡＳ1 유전자 파쇄 효모 균주 및 이를 이용한 재조합 단백질의 생산 방법 公开/授权日：2015-03-11