基本信息:
- 专利标题： 재조합 ＡＤＡＭＴＳ13 및 기타 단백질을 정제하는 방법, 그리고 이들의 조성물
- 专利标题（英）：Methods of purifying recombinant adamts13 and other proteins and compositions thereof
- 专利标题（中）：纯化重组ADAMTS13及其他蛋白质及其组合物的方法
- 申请号：KR1020127004995 申请日：2010-08-02
- 公开(公告)号：KR1020120053013A 公开(公告)日：2012-05-24
- 发明人： 하슬라허마인하르트 , 미테러아르투르 , 피에들러크리스티안 , 마이어크리스타
- 申请人： 박스알타 인코퍼레이티드 , 박스앨타 게엠베하
- 申请人地址： **** Lakeside Drive, Bannockburn, Illinois *****, USA
- 专利权人： 박스알타 인코퍼레이티드,박스앨타 게엠베하
- 当前专利权人： 박스알타 인코퍼레이티드,박스앨타 게엠베하
- 当前专利权人地址： **** Lakeside Drive, Bannockburn, Illinois *****, USA
- 代理人： 양영준; 류현경
- 优先权： US61/230,308 2009-07-31
- 国际申请： PCT/EP2010/061192 2010-08-02
- 国际公布： WO2011012726 2011-02-03
- 主分类号： C07K1/18
- IPC分类号： C07K1/18 ; C07K1/34 ; C12N9/64 ; C12N15/57
In the present invention, from the sample discharge integrin with thrombospondin type 1 motif, 13 - is presented a method for purifying the like and metal protease (ADAMTS13) recombinant protein. The method by contacting the sample with the HA and the chromatographic conditions under which the ADAMTS13 protein to appear in the effluent or supernatant from hydroxyapatite comprising thickening the ADAMTS13 protein. The method may further comprise a tandem chromatography with a mixed form cation-exchange / hydrophobic interaction resin to bind to ADAMTS13 protein. Additional optional steps of ultrafiltration is followed by a / diafiltration, anion exchange chromatography, cation exchange chromatography, and the virus inactivation. Further, in the present invention it is provided a method of disabling the viral protein contaminants in a sample, wherein said protein is immobilized on a support. Further, in the present invention it is presented the composition of ADAMTS13 prepared according to these methods.
公开/授权文献:
- KR101769634B1 재조합 ＡＤＡＭＴＳ13 및 기타 단백질을 정제하는 방법, 그리고 이들의 조성물 公开/授权日：2017-08-18