基本信息:
- 专利标题： 나노입자가 융합된 ＦＲＥＴ 센서 및 이를 이용한 파이토에스트로겐을 고감도로 검출하는 방법
- 专利标题（英）：Fret sensor fused nano-particles and detecting method for phytoestrogen using the same
- 专利标题（中）：FRET传感器熔融纳米颗粒和使用它的植物色素的检测方法
- 申请号：KR1020100055696 申请日：2010-06-11
- 公开(公告)号：KR1020110135775A 公开(公告)日：2011-12-19
- 发明人： 우의전 , 아티 , 정상전 , 이명규 , 이승구
- 申请人： 한국생명공학연구원
- 申请人地址： ***, Gwahak-ro, Yuseong-gu, Daejeon, (***-***), Republic of Korea
- 专利权人： 한국생명공학연구원
- 当前专利权人： 한국생명공학연구원
- 当前专利权人地址： ***, Gwahak-ro, Yuseong-gu, Daejeon, (***-***), Republic of Korea
- 代理人： 손민
- 主分类号： G01N33/74
- IPC分类号： G01N33/74 ; G01N33/52 ; G01N21/64
摘要：
PURPOSE: A nanoparticle-fused FRET sensor is provided to accurately detect phytoestrogen and to be properly applied in food and medical fields. CONSTITUTION: An FRET(fluorescence resonance energy transfer) sensor contains nanoparticles fused to estrogen receptor ligand binding domain containing a donor fluorescent protein and receptor fluorescent protein. The donor fluorescent protein and receptor fluorescent protein are linked to N-terminal and C-terminal of the estrogen receptor ligand binding domain, respectively. The nanoparticles are quantum dots containing carboxylic groups, polymers, magnetic material, or gold. The nanoparticles are a carboxylated polystyrene nanoparticles.
摘要（中）：
目的：提供纳米颗粒融合的FRET传感器，以准确检测植物雌激素，并适用于食品和医疗领域。 构成：FRET（荧光共振能量转移）传感器含有与含有供体荧光蛋白和受体荧光蛋白的雌激素受体配体结合结构域融合的纳米颗粒。 供体荧光蛋白和受体荧光蛋白分别与雌激素受体配体结合结构域的N末端和C-末端连接。 纳米颗粒是含有羧基，聚合物，磁性材料或金的量子点。 纳米颗粒是羧化聚苯乙烯纳米颗粒。
公开/授权文献:
- KR101631581B1 나노입자가 융합된 ＦＲＥＴ 센서 및 이를 이용한 파이토에스트로겐을 고감도로 검출하는 방법 公开/授权日：2016-06-17