基本信息:
- 专利标题： 재설계 유전자회로를 이용한 목적 효소 활성의 신규 탐색 및 정량 방법
- 专利标题（英）：Novel method for detecting and quantitating target enzyme activity using artificial genetic circuitry
- 专利标题（中）：使用人工遗传电路检测和定量目标酶活性的新方法
- 申请号：KR1020100054100 申请日：2010-06-08
- 公开(公告)号：KR1020100131955A 公开(公告)日：2010-12-16
- 发明人： 이승구 , 나유진 , 최수림 , 송재준 , 최종현
- 申请人： 한국생명공학연구원
- 申请人地址： ***, Gwahak-ro, Yuseong-gu, Daejeon, (***-***), Republic of Korea
- 专利权人： 한국생명공학연구원
- 当前专利权人： 한국생명공학연구원
- 当前专利权人地址： ***, Gwahak-ro, Yuseong-gu, Daejeon, (***-***), Republic of Korea
- 代理人： 이처영
- 优先权： KR1020090050596 2009-06-08
- 主分类号： C12Q1/25
- IPC分类号： C12Q1/25 ; C12N15/52 ; C12N15/63 ; C40B40/08
摘要：
PURPOSE: A novel method for searching and quantitating target enzyme activation is provided to quickly sense and quantitate useful genes with high sensitivity. CONSTITUTION: A method for searching target enzyme activity using a genetic circuit comprises: a step of preparing a phenol compound sensing artificial genetic circuit containing a gene encoding phenol compound lyase control protein, reporter gene and gene expression control region; a step of preparing clone or gene library having a gene encoding detection enzyme; a step of introducing the artificial genetic circuit to a host microorganism; a step of treating the recombinant microorganism with phenol releasing-compound; and a step of sensing activation of induced reporter protein.
摘要（中）：
目的：提供一种新颖的方法，用于搜索和定量靶酶活化，以快速感测和定量高灵敏度的有用基因。 构成：使用遗传回路搜索靶酶活性的方法包括：制备含有编码酚化合物裂解酶控制蛋白基因，报告基因和基因表达控制区的基因的人工遗传回路的酚类化合物的步骤; 制备具有编码检测酶的基因的克隆或基因文库的步骤; 将人造遗传回路引入宿主微生物的步骤; 用酚释放化合物处理重组微生物的步骤; 以及感测报道蛋白激活的步骤。
公开/授权文献:
- KR101222056B1 재설계 유전자회로를 이용한 목적 효소 활성의 신규 탐색 및 정량 방법 公开/授权日：2013-01-14