基本信息:
- 专利标题： 적혈구에 활성 성분을 내재화시키는 용해/재봉합 방법 및 장치
- 专利标题（英）：Lysis/resealing process and device for incorporating an active ingredient in erythrocytes
- 专利标题（中）：用于在红细胞中加入活性成分的LYSIS / RESEALING PROCESS AND DEVICE
- 申请号：KR1020077004994 申请日：2005-08-04
- 公开(公告)号：KR1020070058478A 公开(公告)日：2007-06-08
- 发明人： 고드프랭,얀느
- 申请人： 에리테끄 파르마
- 申请人地址： ** avenue Rockefeller, F-***** Lyon, France
- 专利权人： 에리테끄 파르마
- 当前专利权人： 에리테끄 파르마
- 当前专利权人地址： ** avenue Rockefeller, F-***** Lyon, France
- 代理人： 리앤목특허법인
- 优先权： FR04 08667 2004-08-05
- 国际申请： PCT/IB2005/002323 2005-08-04
- 国际公布： WO2006016247 2006-02-16
- 主分类号： A61K9/50
- IPC分类号： A61K9/50 ; A61P35/02 ; A61K31/6615 ; A61K38/50
摘要：
Lysis/resealing process and device for incorporating an active ingredient, in particular asparaginase or inositol hexaphosphate, in erythrocytes ABSTRACT Lysis/resealing process for preparing erythrocytes which contain an active ingredient, the process comprising the following steps: (1) - placing a globular concentrate in suspension in an isotonic solution having a haematocrit level which is equal to or greater than 65 %, with refrigeration at from + 1 to + 8 °C, (2) - measuring the osmotic fragility based on a sample of erythrocytes from that same globular concentrate, preferably on a sample of the suspension obtained in step (1), (3) - lysis and internalisation procedure of the active ingredient, inside the same chamber, at a temperature which is constantly maintained at from + 1 to + 8 °C, comprising allowing the erythrocyte suspension having a haematocrit level which is equal to or greater than 65 % and a hypotonic lysis solution which is refrigerated at from + 1 to + 8 °C, to circulate in a dialysis cartridge; the lysis parameters being adjusted in accordance with the osmotic fragility previously measured; and (4) - resealing procedure carried out in a second chamber at a temperature of from + 30 to + 40 °C by means of a hypertonic solution. Figure 1.
摘要（中）：
用于在红细胞中掺入活性成分，特别是天冬酰胺酶或肌醇六磷酸的裂解/再密封方法和装置摘要：用于制备含有活性成分的红细胞的裂解/再密封方法，该方法包括以下步骤：（1） - 将球状浓缩物 在具有等于或大于65％的血细胞比容水平的等渗溶液中悬浮，在+ 1至+ 8℃下冷藏，（2） - 测量基于来自相同球状体的红细胞样品的渗透脆性 浓缩，优选在步骤（1）中获得的悬浮液的样品上，（3） - 在恒定保持在+ 1至+ 8℃的温度下在同一室内的活性成分的裂解和内化过程 包括使血细胞比容水平等于或大于65％的红血球悬浮液和从+ 1至+ 8℃冷冻的低渗裂解溶液至 在透析筒中循环; 裂解参数根据先前测量的渗透脆性进行调整; 和（4） - 在第二室中通过高渗溶液在+ 30至+ 40℃的温度下进行的再密封程序。 图1。
公开/授权文献:
- KR101332733B1 적혈구에 활성 성분을 내재화시키는 용해/재봉합 방법 및 장치 公开/授权日：2013-11-25
信息查询:
EspacenetIPC结构图谱:
| A | 人类生活必需 |
| --A61 | 医学或兽医学；卫生学 |
| ----A61K | 医用、牙科用或梳妆用的配制品 |
| ------A61K9/00 | 以特殊物理形状为特征的医药配制品 |
| --------A61K9/10 | .分散液；乳剂 |
| ----------A61K9/50 | ..微型胶囊 |