基本信息:
- 专利标题： 제조 방법
- 专利标题（英）：Production method
- 专利标题（中）：生产方法
- 申请号：KR1020107006104 申请日：2008-08-19
- 公开(公告)号：KR101637533B1 公开(公告)日：2016-07-11
- 发明人： 유덴,마크 , 코트솝오울오우,에카테리니
- 申请人： 글락소 그룹 리미티드
- 申请人地址： *** Great West Road, Brentford, Middlesex TW* *GS, United Kingdom
- 专利权人： 글락소 그룹 리미티드
- 当前专利权人： 글락소 그룹 리미티드
- 当前专利权人地址： *** Great West Road, Brentford, Middlesex TW* *GS, United Kingdom
- 代理人： 특허법인 남앤드남
- 优先权： US60/956,772 2007-08-20
- 国际申请： PCT/EP2008/060834 2008-08-19
- 国际公布： WO2009024567 2009-02-26
- 主分类号： C12N15/67
- IPC分类号： C12N15/67 ; C07K16/18 ; C12N5/10 ; C12N15/85
The present invention provides a method for reducing the number, the level of pressure necessary to choose appropriate time and amplification cycle takes sikimeuroseo transform the desired codon of the open reading frame to generate a cell line expressing the protein. The method of the present invention through the use of the modified codon (codon adaptation) for the purpose of providing a constant sufficient to yield a short time frame, while saving a lot of time (week) on the cell line development. In addition, the method of the present invention and also produce a cell line using the selection and amplification of the lower concentrations than is possible conventionally achieved. Therefore, the lower level of selection and amplification marker is observed in the final cell line.
公开/授权文献:
- KR1020100065332A 제조 방법 公开/授权日：2010-06-16