The present invention is a bacterial systemic disease causative diagnostic primer sets, and particularly the forward primer of SEQ ID NO: 1 than relates to a diagnostic kit comprising the primer set of the poultry (forward primer) and a reverse primer of SEQ ID NO: 2 (reverse primer) consisting of a primer set that can be made up to diagnose bacterial systemic disease causative agent of E. coli in poultry primers, forward primer of SEQ ID NO: 3 and reverse primer of SEQ ID NO: 4 to diagnose bacterial systemic disease causative agents of rime Pasteurella bacteria in poultry, SEQ ID NO: 5 sets of forward primers and SEQ ID NO least one selected from consisting of primers that can be diagnosed with bacterial systemic disease pathogens of Salmonella in poultry with the reverse primer of 6 one primer, bacterial systemic disease in poultry comprising the primer set Pathogens diagnostic composition, bacterial of the primer to the three kinds of fowl, including using a diagnostic kit comprising a diagnostic kit and a primer set that can be diagnosed with bacterial systemic disease causative agent of fowl selective diagnostic and / or at the same time at least one set systemic disease in E. coli (Escherichia coli, E.coli) organisms, Pasteurella bacteria rime relates to (Riemerella anatipestifer, R. anatipestifer), Salmonella (Salmonella spp.) the diagnostic methods that can be selectively diagnosed as diagnostic and / or at the same time .