The present invention relates to a method for producing silk / hydroxyapatite composite nanofiber scaffold for bone regeneration, more specifically, the step (step 1) for preparing a silk spinning solution; Comprising electrospinning a silk spinning solution prepared in the above step 1 (step 2); Dispersing the nanofibers silk spinning in the step 2 (step 3); (Step 4) for producing a silk nanofiber of the sponge form of the dispersion silk nanofibers dispersed in the above step 3 and lyophilized transport in a predetermined vessel in three dimensions; The human body similar to the solution of Step 4. The three-dimensionally in a sponge form of a 5-fold to 15-fold the silk nanofiber concentration dispersion prepared in (simulated body fluid, SBF) step by immersion in a composite coating with hydroxyapatite (Step 5 ); And to a method of manufacturing a bone regeneration silk / hydroxyapatite composite nanofiber support substrate comprises a step (step 6) to form an air gap in the silk fiber composite nano-coating in the above step 5 as the hydroxyapatite. According to the present invention, 5 times to 15 times the human body similar to the solution for use by normal temperature and normal pressure conditions silk protein damage short plasma treatment or surface additional silk nanofibers to hydroxyapatite without performing processes such as processing on without at the concentration It can be efficiently coated with the support, and can lower the degree of crystallinity as compared to hydroxyapatite to produce the silk / hydroxyapatite composite nanofiber of the support similar to the natural bone.