While using the easily obtained reagents in high yields, the detection method and quantification method of the detection target to be detected can be properly detected and quantified, the kit, as well as to provide a process for the preparation of the reagents.
Method for detecting a target in a sample includes a first bound substance 10 and the first affinity substance 13 relative to the first material and the detection target 50 containing a stimuli-responsive substance 11 is bound, hydrophilic or a second bound substance 20 and the second material 21 having a portion of the organic charge and a second affinity substance 23 with respect to the detection target 50 is bonded, by mixing the sample, the mixture is stimulus responsive substance 11 Place under conditions to aggregate, comprising the step of determining the presence or absence of dispersion or event that correlates with that of the stimulus responsive substance 11. The second material 21 has a specific gravity are those containing particles is 1.4 or more, the first affinity substance 13 and the second affinity substance 23, in different sites of the detection target 50, at the same time detected It can be attached to the 50.