The present invention, biological material, for example, solubilization of proteins or cell membranes, digestion, preparation, analysis, and provides a surfactant compound of formula I~IX which can be used in the process to assist / or characterization. The compounds may also help in particular the recovery of peptides generated during protein digestion in gel digestion protocol. Additionally, the compounds, without interfering with the downstream sample preparation steps and mass spectrometry, it is possible to improve the enzymatic protein deglycosylation. The compounds, acid may be particularly useful as a heat or digestive aids which can be decomposed by a combination thereof. When the surfactant is decomposed, can be easily separated from the isolated sample, the sample can be analyzed without and / or preventing the sensitivity of the various analytical techniques.
.TECHNICAL