基本信息:
- 专利标题： Simplified measurement method of enterohemorrhagic escherichia coli
- 专利标题（中）：肠易激综合征的简化测量方法
- 申请号：JP2009021918 申请日：2009-02-02
- 公开(公告)号：JP2009145357A 公开(公告)日：2009-07-02
- 发明人： SATO YOSHIHIRO
- 申请人： Denka Seiken Co Ltd , デンカ生研株式会社
- 专利权人： Denka Seiken Co Ltd,デンカ生研株式会社
- 当前专利权人： Denka Seiken Co Ltd,デンカ生研株式会社
- 优先权： JP2009021918 2009-02-02
- 主分类号： G01N33/569
- IPC分类号： G01N33/569 ; G01N33/543 ; G01N33/545
摘要：
PROBLEM TO BE SOLVED: To provide a detecting method of enterohemorrhagic escherichia coli producing verotoxin.
SOLUTION: This detecting method of enterohemorrhagic escherichia coli includes the step to react anti-verotoxin antibody sensitized particles with escherichia coli fungus body, and to measure an aggregation level of a reactant mixture from a change in absorbance or change in scattered light intensity. It also includes the step to destroy the cell wall of the escherichia coli fungus body, and uses an analytical measuring device to perform measurement. It also includes the step to process the escherichia coli fungus body by using polymyxin B, and to react anti-verotoxin antibody sensitized particles with escherichia coli fungus body in which verotoxin is bound to its surface, to measure the aggregation level of the reactant mixture.
COPYRIGHT: (C)2009,JPO&INPIT
摘要（中）：
SOLUTION: This detecting method of enterohemorrhagic escherichia coli includes the step to react anti-verotoxin antibody sensitized particles with escherichia coli fungus body, and to measure an aggregation level of a reactant mixture from a change in absorbance or change in scattered light intensity. It also includes the step to destroy the cell wall of the escherichia coli fungus body, and uses an analytical measuring device to perform measurement. It also includes the step to process the escherichia coli fungus body by using polymyxin B, and to react anti-verotoxin antibody sensitized particles with escherichia coli fungus body in which verotoxin is bound to its surface, to measure the aggregation level of the reactant mixture.
COPYRIGHT: (C)2009,JPO&INPIT
待解决的问题：提供产生肠毒素的大肠杆菌生产的毒素的检测方法。 解决方案：这种肠出血大肠杆菌的检测方法包括使大肠杆菌真菌体内的抗痘苗毒素抗体致敏颗粒反应的步骤，从吸光度的变化或散射光强度的变化来测定反应物混合物的凝集水平 。 它还包括破坏大肠杆菌真菌体的细胞壁的步骤，并使用分析测量装置进行测量。 它还包括通过使用多粘菌素B处理大肠杆菌真菌体的步骤，并使抗毒素毒素抗体致敏颗粒与其中毒素与其表面结合的大肠杆菌真菌体反应，以测量反应物混合物的聚集水平。 版权所有（C）2009，JPO＆INPIT
公开/授权文献:
信息查询:
EspacenetIPC结构图谱:
| G | 物理 |
| --G01 | 测量；测试 |
| ----G01N | 借助于测定材料的化学或物理性质来测试或分析材料 |
| ------G01N33/00 | 利用不包括在G01N1/00至G01N31/00组中的特殊方法来研究或分析材料 |
| --------G01N33/48 | .生物物质，例如血、尿（G01N33/02至G01N33/14，G01N33/26，G01N33/44，G01N33/46优先；种子发芽能力测定入A01C1/02）；血球计数器 |
| ----------G01N33/483 | ..生物物质的物理分析 |
| ------------G01N33/53 | ...免疫测定法；生物特有的结合方法的测定；相应的生物物质 |
| --------------G01N33/569 | ....用于微生物，例如原生动物、细菌、病毒 |