发明公开
EP4180810A1 FLUORESCENT CROSS-LINKED RNASE H MUTANT CONJUGATE, MIRNA COMBINATION AND APPLICATION THEREOF
审中-实审
基本信息:
- 专利标题： FLUORESCENT CROSS-LINKED RNASE H MUTANT CONJUGATE, MIRNA COMBINATION AND APPLICATION THEREOF
- 申请号：EP21838258.8 申请日：2021-07-12
- 公开(公告)号：EP4180810A1 公开(公告)日：2023-05-17
- 发明人： WANG, Yiran
- 申请人： Shanghai Miran Biotech Co., Ltd.
- 申请人地址： CN Shanghai 201203 5L, No. 780, Cailun Rd, Pudong New District
- 代理机构： Dehns
- 优先权： CN202011400346 20201201
- 国际公布： WO2022007970 20220113
- 主分类号： G01N33/533
- IPC分类号： G01N33/533 ; C12N9/22 ; C12Q1/6886 ; G16B40/10 ; G16B20/50
摘要：
A fluorescent cross-linked RNase H mutant conjugate, a miRNA combination and an application thereof. The fluorescent cross-linked RNase H mutant conjugate (i) is as represented by RNase Hv-(Lx-SH-F)n or (ii) comprises an RNase Hv-Lx-ligand and receptor-F, wherein the ligand can bind to the receptor, RNase Hv is an RNase H mutant, which can bind to RNA or RNA-DNA hybrid strands, but cannot cleave RNA; L is a linker, and x is 1-10; SH is an amino acid containing a sulfhydryl group; F is a luminescent functional group, and n is 1-7. The fluorescent cross-linked RNase H mutant conjugate can directly recognize DNA/RNA hybrid strands and can be converted to generate detectable signals without PCR amplification, and can be applied to sensitively and quickly detect RNA.
IPC结构图谱:
| G | 物理 |
| --G01 | 测量；测试 |
| ----G01N | 借助于测定材料的化学或物理性质来测试或分析材料 |
| ------G01N33/00 | 利用不包括在G01N1/00至G01N31/00组中的特殊方法来研究或分析材料 |
| --------G01N33/48 | .生物物质，例如血、尿（G01N33/02至G01N33/14，G01N33/26，G01N33/44，G01N33/46优先；种子发芽能力测定入A01C1/02）；血球计数器 |
| ----------G01N33/483 | ..生物物质的物理分析 |
| ------------G01N33/53 | ...免疫测定法；生物特有的结合方法的测定；相应的生物物质 |
| --------------G01N33/531 | ....免疫化学试验物质的制备 |
| ----------------G01N33/532 | .....示踪免疫化学物品的制备 |
| ------------------G01N33/533 | ......带有荧光标记的 |