基本信息:
- 专利标题： DNA-BINDING PROTEIN USING PPR MOTIF, AND USE THEREOF
- 申请号：EP20151941.0 申请日：2014-04-22
- 公开(公告)号：EP3696186A1 公开(公告)日：2020-08-19
- 发明人： Yamamoto, Takashi , Sakuma, Tetsushi , Nakamura, Takahiro , Yagi, Yusuke , Okawa, Yasuyuki
- 申请人： Kyushu University, National University Corporation , Hiroshima University
- 申请人地址： 10-1, Hakozaki, 6-chome Higashi-ku Fukuoka-shi Fukuoka 812-8581 JP
- 专利权人： Kyushu University, National University Corporation,Hiroshima University
- 当前专利权人： Kyushu University, National University Corporation,Hiroshima University
- 当前专利权人地址： 10-1, Hakozaki, 6-chome Higashi-ku Fukuoka-shi Fukuoka 812-8581 JP
- 代理机构： Hoffmann Eitle
- 优先权： JP2013089840 20130422
- 主分类号： C07K4/10
- IPC分类号： C07K4/10 ; C07K14/415 ; C07K19/00 ; C12N9/16 ; C12Q1/68 ; C12N9/22 ; C12N15/82
摘要：
The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an α-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an α-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. "ii" (-2) A.A. contained in L of the formula 1, the aforementioned object could be achieved.
(Helix A)-X-(Helix B)-L (Formula 1)
(Helix A)-X-(Helix B)-L (Formula 1)
公开/授权文献:
- EP3696186B1 DNA-BINDING PROTEIN USING PPR MOTIF, AND USE THEREOF 公开/授权日：2024-09-11