![METHOD FOR MULTIPLEX NUCLEIC ACID ANALYSIS](/ep/2016/04/13/EP2893034A4/abs.jpg.150x150.jpg)
基本信息:
- 专利标题: METHOD FOR MULTIPLEX NUCLEIC ACID ANALYSIS
- 专利标题(中):方法进行多重分析NUCLEIC
- 申请号:EP12883998 申请日:2012-09-10
- 公开(公告)号:EP2893034A4 公开(公告)日:2016-04-13
- 发明人: JIANG ZHENGWEN , YU FENG , LI CAIHUA
- 申请人: GENESKY DIAGNOSTICS SUZHOU INC , SHANGHAI GENESKY BIOTECHNOLOGIES INC
- 专利权人: GENESKY DIAGNOSTICS SUZHOU INC,SHANGHAI GENESKY BIOTECHNOLOGIES INC
- 当前专利权人: GENESKY TECHNOLOGIES (SUZHOU) INC., SUZHOU, CN
- 当前专利权人地址: GENESKY TECHNOLOGIES (SUZHOU) INC., SUZHOU, CN
- 优先权: CN2012081212 2012-09-10
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; G01N21/64
摘要:
Here provided is a method for multiplex nucleic acid analysis. The method includes steps of hybridizing sets of probes to target nucleic acids in a sample, ligating the hybridized probes, amplifying the ligated probes, and assaying the amplification products to determining the presence, absence, or quantity of the target nucleic acids in the sample. The multiplexity is made available in part by adding detectable moieties and inserting stuffer sequences in primers so that amplification products may be identified on the basis of the detectable moieties and fragment sizes. Also provided is a sensitive method of detecting small copy number changes by measuring the copy number of a plurality of target sites in the nucleic acid in a test sample in comparison to a control sample and then determining the copy number of the nucleic acid based on the measured copy number of the plurality of target sites. Further provided is a kit for multiplex nucleic acid analysis and for small copy number change determination.