基本信息:
- 专利标题： METHOD FOR EVALUATING SPECIFIC INCORPORATION OF D-GLUCOSE INTO CELLS
- 专利标题（中）：程序评估D-葡萄糖在细胞中特定的存储的
- 申请号：EP09805069.3 申请日：2009-08-07
- 公开(公告)号：EP2325327B1 公开(公告)日：2013-04-24
- 发明人： YAMADA Katsuya , MATSUOKA Hideaki , TESHIMA Tadashi , YAMAMOTO Toshihiro
- 申请人： Hirosaki University , National University Corporation Tokyo University of Agriculture and Technology
- 申请人地址： 1, Bunkyo-cho Hirosaki-shi, Aomori 036-8560 JP
- 专利权人： Hirosaki University,National University Corporation Tokyo University of Agriculture and Technology
- 当前专利权人： ORBIO CORP., JP
- 当前专利权人地址： ORBIO CORP., JP
- 代理机构： Walcher, Armin
- 优先权： JP2008205708 20080808
- 国际公布： WO2010016587 20100211
- 主分类号： C12Q1/54
- IPC分类号： C12Q1/54 ; C07H1/00 ; C07H5/06 ; C07H13/00 ; C07H17/00 ; C12Q1/04 ; C12Q1/02 ; G01N33/50
摘要：
An object of the present invention is to provide a method for accurately evaluating the specific incorporation of D-glucose into cells. The present invention as a means for achieving the object is characterized by comprising contacting a D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells and an L-glucose derivative that has a fluorescent chromophore in the molecule with different cells in the same cell strain to be evaluated, respectively, comparing the fluorescence emitted by the D-glucose derivative that has a fluorescent chromophore in the molecule and is specifically incorporated into cells with the fluorescence emitted by the L-glucose derivative that has a fluorescent chromophore in the molecule, and evaluating the specific incorporation of D-glucose into cells relative to L-glucose by taking the difference between the two kinds of fluorescence intensities.