基本信息:
- 专利标题： Sample preparation method incorporating an alkaline shock
- 专利标题（中）：Probenherstellungsverfahren mit einemAlkalistoß
- 申请号：EP10006190.2 申请日：2006-02-17
- 公开(公告)号：EP2243832A2 公开(公告)日：2010-10-27
- 发明人： Gao, Kui , Becker, Michael M. , Wu, Wen , Linnen, Jeffrey M.
- 申请人： Gen-Probe Incorporated
- 申请人地址： 10210 Genetic Center Drive San Diego, CA 92121 US
- 专利权人： Gen-Probe Incorporated
- 当前专利权人： Gen-Probe Incorporated
- 当前专利权人地址： 10210 Genetic Center Drive San Diego, CA 92121 US
- 代理机构： Lloyd, John Scott
- 优先权： US654199P 20050218; US669192P 20050406
- 主分类号： C12N15/10
- IPC分类号： C12N15/10
摘要：
Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invention method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.
摘要（中）：
制备适合用于随后的体外核酸扩增反应的生物样品的方法。 该方法涉及对碱性条件的快速，瞬时暴露，其可以通过将碱性溶液与包含洗涤剂的pH缓冲溶液和待测试的生物样品混合使用体外扩增来存在特定的核酸物种来实现。 本发明方法有利地可以改进某些靶核酸的检测，而不会显着损害他人的可检测性。 该方法对于同时制备可用于多重扩增反应的RNA和DNA模板特别有用。
公开/授权文献:
- EP2243832A3 Sample preparation method incorporating an alkaline shock 公开/授权日：2011-01-05