The invention relates to a method for catalytic production of L-dopa by an immobilized enzyme, wherein the method is characterized by comprising the steps: (1) selecting a single colony, inoculating the single colony into a test tube containing an LB culture medium, adding ampicillin (100 mg/L) at the temperature of 30-37 DEG C, at the speed of 220 rpm, culturing for 12-16 h, and thus obtaining primary seeds; (2) inoculating the primary seeds into a shaking flask containing a fermentation culture medium for 10-12 h; (3) centrifuging, collecting bacteria to obtain the bacteria, squeezing and breaking cells, adding a chitosan solution, flocculating, centrifuging at high speed to obtain a supernatant enzyme liquid, then adding an immobilized carrier EP200, stirring and mixing at the temperature of 25 DEG C for 12-24 h, carrying out three-legged centrifugal collection of the immobilized enzyme, washing for multiple times with a 50 mM potassium phosphate buffer solution with the pH of 8.0,and filtering to obtain the immobilized enzyme; and (4) adding 20 g of the immobilized enzyme into a substrate solution, stirring evenly, and carrying out sealed oscillation reaction at the temperature of 25 DEG C, wherein the substrate solution comprises 14-16 g/L of sodium pyruvate, 10-12 g/L of catechol, 40-45 g/L of ammonium chloride, 2-5 g/L of sodium sulfite and 1-3 g/L of EDTA, and the pH is adjusted to 7.5-8.5.