基本信息:
- 专利标题： 特异识别猪Tert位点的sgRNA及其编码DNA和应用
- 专利标题（英）：SgRNA (small guide Ribonucleic Acid) for specifically identifying swine Tert (Telomerase Reverse Transcriptase) locus and encoding DNA (Deoxyribonucleic Acid) and application thereof
- 申请号：CN201910071828.9 申请日：2019-01-24
- 公开(公告)号：CN109679958A 公开(公告)日：2019-04-26
- 发明人： 李奎 , 阮进学 , 徐奎 , 张秀玲 , 裴杨莉 , 李华
- 申请人： 佛山科学技术学院
- 申请人地址： 广东省佛山市南海区狮山镇仙溪水库西路佛山科学技术学院
- 专利权人： 佛山科学技术学院
- 当前专利权人： 佛山科学技术学院
- 当前专利权人地址： 广东省佛山市南海区狮山镇仙溪水库西路佛山科学技术学院
- 代理机构： 广州嘉权专利商标事务所有限公司
- 代理人： 王国标
- 主分类号： C12N15/113
- IPC分类号： C12N15/113 ; C12N15/54
The invention provides sgRNA (small guide Ribonucleic Acid) for specifically identifying a swine Tert (Telomerase Reverse Transcriptase) locus. The sgRNA is formed by pTert-exon1-sgRNA and pTert-exon2-sgRNA, wherein a nucleotide sequence which is responsible for target fragments in the pTert-exon1-sgRNA is SEQ ID NO.1; a nucleotide sequence which is responsible for target fragments in the pTert-exon2-sgRNA is SEQ ID NO.2. The sgRNA for the swine Tert locus is strong in specificity. The sgRNA for the swine Tert locus can utilize CRISPR/Cas9 gene editing system and a bifluorescence enrichment method and is capable of efficiently realizing large-fragment knockout of the swine Tert gene, the homozygote efficiency can be up to 50 percent, and preliminary technical support is provided for follow-up research on swine Tert gene functions.
公开/授权文献:
- CN109679958B 特异识别猪Tert位点的sgRNA及其编码DNA和应用 公开/授权日：2022-04-26