基本信息:
- 专利标题： 一种靶向骨组织表面俘获间充质干细胞的融合蛋白及应用
- 专利标题（英）：Fusion protein for capturing mesenchymal stem cells on surfaces of targeting bone tissues and application thereof
- 申请号：CN201811426541.5 申请日：2018-11-27
- 公开(公告)号：CN109535261A 公开(公告)日：2019-03-29
- 发明人： 赵彦涛 , 衷鸿宾 , 侯树勋 , 韩丽伟 , 胡先同 , 白玉龙 , 张看 , 李利 , 张春丽 , 章亚东
- 申请人： 中国人民解放军总医院第一附属医院
- 申请人地址： 北京市海淀区阜成路51号
- 专利权人： 中国人民解放军总医院第一附属医院
- 当前专利权人： 中国人民解放军总医院第四医学中心
- 当前专利权人地址： 北京市海淀区阜成路51号
- 代理机构： 北京慕达星云知识产权代理事务所
- 代理人： 李冉
- 主分类号： C07K19/00
- IPC分类号： C07K19/00 ; C07K1/06 ; A61L27/22 ; A61L27/54
The present invention discloses a fusion protein for capturing mesenchymal stem cells on surfaces of targeting bone tissues, and also discloses a preparation method thereof. The preparation method comprises the following steps: (1) a Fmoc-Ser(tBu)-Wang resin is used as a start, after Fmoc is removed, a coupling agent is used to gradually couple protect amino acids, and finally, protect mimetic peptides are coupled to obtain a fusion protein-Wang; (2) peptide cutting and diethyl ether precipitation are conducted to obtain a fusion protein crude product; and (3) the fusion protein crude productis dissolved in water, pH is adjusted to 3-5, and a precipitate is collected to obtain the fusion protein. The fusion protein has good affinity with an osteogenic region, can stably bind with the osteogenic region, captures mesenchymal stem cells on the osteogenic region, promotes differentiation of the mesenchymal stem cells into osteoblasts and other bone tissue cells, and thus improves repair quality of the osteogenic region.
公开/授权文献:
- CN109535261B 一种靶向骨组织表面俘获间充质干细胞的融合蛋白及应用 公开/授权日：2021-03-23