The invention relates to the technical field of mesoporous molecular sieves and discloses a preparation method for an amino-functionalized mesoporous molecular sieve film. The preparation method comprises the following steps: uniformly mixing poly(dimethoxysilane), amino silane, a template and a photo base generator and smearing the mixture to a substrate, and irradiating the mixture with a high pressure mercury lamp to form an organic silicon film; and soaking the organic silicon film in 40 DEG C deionized water to remove the template to obtain the amino-functionalized mesoporous molecular sieve film. Compared with the prior art, the organic silicon mesoporous molecular sieve film containing a lot of amino in ducts is quickly prepared by means of a photoinduced alkali sol-gel method by one step, so that the film can adsorb heavy metal ions by means of coordination or ionic bond action. According to the method, no organic solvents are used in the preparation process, and the prepared film is high in ability of adsorbing heavy metal ions (the adsorbing removal rate of cobalt ions can reach 97%), and can be simply separated and regenerated and used.