The invention relates to heart-tonifying peptide fruit juice compounded from peptide and apple juice and a preparation method thereof, belongs to the technical field of liquid processing and providesa novel functional peptide fruit juice food for the market. Corn peptide powder, soybean peptide powder, almond peptide powder, ginseng peptide powder, ganoderma lucidum peptide powder, agrocybe aegirit peptide powder, long-rocked mushroom peptide powder, walnut peptide powder, peanut peptide powder, white fungus peptide powder and garlic peptide powder with the effect of tonifying the heart are used as main materials, polysaccharides, vitamins, trace elements, essential oil and the like are used as auxiliary materials, and the main materials and the auxiliary materials are prepared into mixedpeptide powder by weighing, mixing, granulation, drying and packaging. Pure apple juice is taken and filled into a fresh-keeping can, the peptide fruit juice is prepared by mixing the mixed peptide powder and the apple juice in ratio of 1:50, and the peptide fruit juice has the effect of supplementing nutrition.