基本信息:
- 专利标题： 一种检测隐秘杆菌的实时定量LAMP引物、试剂盒及方法
- 专利标题（英）：Real-time quantitative LAMP (Loop-Mediated Isothermal Amplification) primer, kit and method for detecting arcanobacterium
- 申请号：CN201710313040.5 申请日：2017-05-05
- 公开(公告)号：CN107130022A 公开(公告)日：2017-09-05
- 发明人： 李军 , 吴翠兰 , 彭昊 , 冯世文 , 潘艳 , 陶立 , 马春霞 , 谢永平 , 钟舒红 , 胡帅 , 杨威 , 陈泽祥
- 申请人： 广西壮族自治区兽医研究所
- 申请人地址： 广西壮族自治区南宁市西乡塘区友爱北路51号
- 专利权人： 广西壮族自治区兽医研究所
- 当前专利权人： 广西壮族自治区兽医研究所
- 当前专利权人地址： 广西壮族自治区南宁市西乡塘区友爱北路51号
- 代理机构： 广州市红荔专利代理有限公司
- 代理人： 李彦孚; 何承鑫
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12Q1/04 ; C12N15/11 ; C12R1/01
The invention discloses a real-time quantitative LAMP (Loop-Mediated Isothermal Amplification) primer, a kit and a method for detecting arcanobacterium. The primer is shown as SEQ ID NO: 1-4; the kit comprises the LAMP primer, 2X reaction buffer, Bst DNA polymerase, ultra-pure water and arcanobacterium DNA. The method comprises the steps: preparation of materials, design and synthesis of the LAMP primer, extraction of a reaction template, establishment of an LAMP reaction system, and specific detection, sensitivity detection and real-time quantitative detection by adopting the LAMP detection method. The specific detection and sensitivity detection prove that the LAMP detection method provided by the invention is capable of monitoring the reaction in real time and performing quantitative detection on a copy number of the arcanobacterium, the detection result can be rapidly and accurately obtained, and convenience is brought to simple and rapid detection of the arcanobacterium.