The invention discloses application of gibberella intermedia QJP2 fermentation extract to prevention and control of plant inset pests and pathogenic nematodes and belongs to the field of microbial pesticide. A strain is preserved in China General Microbiological Culture Collection Center (CGMCC) and the preservation number is CGMCC No. 13199. The gibberella intermedia QJP2 fermentation extract is obtained by fermenting the strain through a shaking table and extracting with ethyl acetate and has relatively insecticidal activity on the plant inset pests including Plutela xylostella and the like; the fatality rate reaches 85.6+/-1.3 percent when the concentration of the gibberella intermedia QJP2 fermentation extract is 1.0mg/mL; moreover, the gibberella intermedia QJP2 fermentation extract also has relatively good inhibition activity on the root knot nematode disease of tomatoes, and the fatality rates of 50-time and 100-time diluents of the gibberella intermedia QJP2 fermentation extract on the root knot nematode disease of the tomatoes reach 95 percent and 81 percent respectively. Therefore, the gibberella intermedia QJP2 fermentation extract can be used for preparing the microbial pesticide for preventing and controlling the plant inset pests and the pathogenic nematodes; compared with chemically synthetized pesticide, the microbial pesticide also has the advantages of environmental friendliness, low toxin to human and livestock and the like and has a relatively good application prospect.