The invention relates to the technical field of medical equipment, and in particular relates to a vacuum blood sedimentation tube of a full-automatic device. The invention adopts a technical scheme: the vacuum blood sedimentation tube of the full-automatic device comprises a cylindrical vacuum tube body and a seal cap, wherein an isolation layer is arranged between the interiors of the seal cap and the vacuum tube body, an arc isolation layer insertion opening is formed in the center of an upper surface of the isolation layer, a lower surface of the isolation layer is also provided with a guide tube, an inner surface of the guide tube is provided with a triangular guide groove, two clamping ends made of a rubber material are arranged at a position, adjacent to the seal cap, of the vacuum tube body, a clamping end surface of each clamping end is a plane, and each clamping end is also provided with a convenient round corner convenient in clamping. The vacuum blood sedimentation tube of the full-automatic device has the advantages that the utilization rate of a collected blood sample and the blood sample detection efficiency are increased, so that the efficiency is higher and more convenient can be achieved when the medical personnel performs the corresponding blood detection, and the pain of patients can be alleviated.