The invention discloses graphene conductive ink as well as a preparing method and an application thereof. The preparing method comprises the following steps: 1, ultrasonically dispersing a dispersant and a graphite flake in a first solution so as to obtain a mixture A; 2, carrying out first centrifuging on the mixture A, and carrying out secondary centrifuging on upper-layer dispersion liquid which is obtained after first centrifuging so as to obtain precipitates B; 3, dispersing the precipitates B in a second solvent so as to obtain dispersion liquid C; 4, adding solvents and additives for the conductive ink into the dispersion liquid C; and 5, removing the second solvent so as to obtain the graphene conductive ink. In the method, the graphene is obtained through in-situ preparation, so that the influence of graphene flaws on conductive performance is avoided. The graphene conductive ink which is high in concentration and high in conductivity can be obtained.