
基本信息:
- 专利标题: 产3-羟基丙酸的重组谷氨酸棒杆菌、其构建方法及应用
- 申请号:CN201610474970.4 申请日:2016-06-24
- 公开(公告)号:CN105950529B 公开(公告)日:2019-09-20
- 发明人: 陈振 , 刘德华
- 申请人: 清华大学 , 东莞深圳清华大学研究院创新中心
- 申请人地址: 北京市海淀区清华园北京100084-82信箱
- 专利权人: 清华大学,东莞深圳清华大学研究院创新中心
- 当前专利权人: 广东清大智兴生物技术有限公司
- 当前专利权人地址: 北京市海淀区清华园北京100084-82信箱
- 代理机构: 北京路浩知识产权代理有限公司
- 代理人: 王文君
- 主分类号: C12N1/21
- IPC分类号: C12N1/21 ; C12N15/77 ; C12P7/42 ; C12R1/15
The invention relates to a 3-hydracrylic-acid-producing recombinant Corynebacterium glutamicum strain, and a construction method and application thereof. The construction method of the recombinant Corynebacterium glutamicum strain comprises the following steps: in a Corynebacterium glutamicum strain, overexpressing an endogenous dihydroxyacetone phosphate phosphatase gene hdpA, and overexpressing a glyceroldehydrogenase gene gldA, a glycerol anhydrase and activating factor gene pduCDEGH and a 3-hydroxypropylaldehyde dehydrogenase gene aldH. When the recombinant strain is used for producing 3-hydracrylic acid by fermentation, the Corynebacterium glutamicum strain can perform fermentation by using different cheap raw materials, thereby further lowering the raw material cost. The 3-hydracrylic-acid-producing recombinant Corynebacterium glutamicum strain solves the problems of biosafety and acid tolerance. The strain in the fermentation process can be used in a feed additive as a product. The method can generate fewer byproducts, so that the separation process of the end product 3-hydracrylic acid is simplified.
公开/授权文献:
- CN105950529A 产3-羟基丙酸的重组谷氨酸棒杆菌、其构建方法及应用 公开/授权日:2016-09-21