The invention provides a tissue culture and rapid propagation method of euryodendron excelsum. The method comprises the following steps: selecting and disinfecting explants, inducing clustered buds, proliferating clustered buds, rooting and culturing, culturing strong seedlings and culturing a seedling bed. Compared with the conventional seedling-raising method of euryodendron excelsum, the method does not adopt euryodendron excels seeds; the new sprout seedlings of the euryodendron excelsum are adopted; few plant raw materials are needed; the euryodendron excelsum is subjected to short-time and mass propagation; the heritable variation of the variety is low; the young seedlings is high in quality; the survival rate of the young seedlings reaches up to 90%; the survival rate of transplanted seedlings reaches up to 80%; after transplanting, the seedlings are excellent in growth vigor; the phenomenon of small seedling is avoided; the problems that the euryodendron excelsum wild resources are increasingly reduced and natural reproduction of the population is difficult can be effectively solved; the technical support is provided for effective protection of euryodendron excelsum serving as a rare and threatened plant.