![一种利用丁基羟基茴香醚促进雨生红球藻生产虾青素的方法](/CN/2016/1/21/images/201610108340.jpg)
基本信息:
- 专利标题: 一种利用丁基羟基茴香醚促进雨生红球藻生产虾青素的方法
- 专利标题(英):Method for promoting haematococcus pluvialis to produce astaxanthin by utilizing butylated hydroxyanisole
- 申请号:CN201610108340.5 申请日:2016-02-27
- 公开(公告)号:CN105648018A 公开(公告)日:2016-06-08
- 发明人: 余旭亚 , 尚敏敏 , 徐军伟 , 李涛 , 赵鹏
- 申请人: 昆明理工大学
- 申请人地址: 云南省昆明市呈贡新区景明南路727号
- 专利权人: 昆明理工大学
- 当前专利权人: 昆明理工大学
- 当前专利权人地址: 云南省昆明市呈贡新区景明南路727号
- 代理机构: 北京国智京通知识产权代理有限公司
- 代理人: 孙文彬
- 主分类号: C12P23/00
- IPC分类号: C12P23/00 ; C07C403/24 ; C12R1/89
The invention discloses a method for promoting haematococcus pluvialis to produce astaxanthin by utilizing butylated hydroxyanisole. Firstly, a haematococcus pluvialis solution is prepared, in the later period of the logarithmic phase of haematococcus pluvialis, the cell concentration reaches 10<6> cells mL<-1>, the solution is diluted with a nitrogen-deficient BBM culture medium until the concentration of the solution reaches 2*10<5> cells mL<-1>, and the solution for induction is obtained; then, a butylated hydroxyanisole mother solution with the concentration being 128 g L<-1> is prepared with DMSO (dimethyl sulfoxide) and added to the diluted induction solution, and accordingly, the concentration of butylated hydroxyanisole reaches 4.95-5.05 mg L<-1>; the solution is cultured, after cells of algae completely turn red, the cells of algae are collected, and at the moment, the astaxanthin accumulation quantity reaches the maximum. The method is simple and feasible, the cost is low, the time consumed when haematococcus pluvialis turns red is greatly shortened, the quantity of astaxanthin is remarkably increased, and the yield of astaxanthin is greatly increased.
公开/授权文献:
- CN105648018B 一种利用丁基羟基茴香醚促进雨生红球藻生产虾青素的方法 公开/授权日:2018-05-22