![一种诱导神经干细胞分化为多巴胺能神经元的方法](/CN/2015/1/179/images/201510899725.jpg)
基本信息:
- 专利标题: 一种诱导神经干细胞分化为多巴胺能神经元的方法
- 专利标题(英):Method for inducing differentiation of neural stem cells (NSCs) into dopaminergic neurons
- 申请号:CN201510899725.3 申请日:2015-12-09
- 公开(公告)号:CN105296429A 公开(公告)日:2016-02-03
- 发明人: 杨洪娜 , 陈恒 , 刘小盾 , 曲廷瑜
- 申请人: 山东省齐鲁干细胞工程有限公司
- 申请人地址: 山东省济南市高新开发区出口加工区港兴三路1109号
- 专利权人: 山东省齐鲁干细胞工程有限公司
- 当前专利权人: 山东省齐鲁干细胞工程有限公司
- 当前专利权人地址: 山东省济南市高新开发区出口加工区港兴三路1109号
- 代理机构: 济南泉城专利商标事务所
- 代理人: 刘丽
- 主分类号: C12N5/0793
- IPC分类号: C12N5/0793
The invention relates to stem cell differentiation technology in the technical field of stem cell treatment and in particular relates to a method for inducing differentiation of neural stem cells (NSCs) into dopaminergic neurons. The method is characterized by culturing 1-2 generation NSCs from the forebrain with a NSC basal culture medium until the diameters of neural spheres are 0.5-1mm, then changing the culture medium to an induction culture medium to enable the cells to grow adhering to the wall and obtaining dopaminergic neurons after culture for 3-10 days, wherein the induction culture medium contains inducers aFGF, TPA, forskolin, pramipexole and BMP-7. The method has the beneficial effects that after the NSCs from the forebrain are cultured by utilizing the induction culture medium, the proportion of the dopaminergic neurons obtained through differentiation of the NSCs is 30%, thus greatly increasing the differentiation rates of the dopaminergic neurons; the dopamine secretion rates of the neurons are increased by more than 20 times; the NSCs from the forebrain are effectively induced into the dopaminergic neurons.