基本信息:
- 专利标题： 植物中流感样病毒颗粒的产生
- 专利标题（英）：Influenza virus-like particle production in plants
- 申请号：CN201480029001.7 申请日：2014-03-28
- 公开(公告)号：CN105247059A 公开(公告)日：2016-01-13
- 发明人： 曼侬·库特里 , 马克-安德烈·蒂奥斯特 , 路易斯-菲利普·维泽纳
- 申请人： 莫迪卡戈公司
- 申请人地址： 加拿大魁北克省
- 专利权人： 莫迪卡戈公司
- 当前专利权人： 阿拉米斯生物技术公司
- 当前专利权人地址： 加拿大魁北克省
- 代理机构： 北京英赛嘉华知识产权代理有限责任公司
- 代理人： 王达佐; 洪欣
- 优先权： 61/806,227 2013.03.28 US; 61/925,852 2014.01.10 US; 61/971,274 2014.03.27 US
- 国际申请： PCT/CA2014/050326 2014.03.28
- 国际公布： WO2014/153674 EN 2014.10.02
- 进入国家日期： 2015-11-19
- 主分类号： C12N15/82
- IPC分类号： C12N15/82 ; A01H5/00 ; A61K39/145 ; A61P31/16 ; A61P37/04 ; C07K14/11 ; C12N15/44 ; C12N7/01 ; C12N7/04
A method of producing a virus like particle (VLP) in a plant comprising modified hemagglutinin is provided. The method comprises introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a modified influenza hemagglutinin (HA) protein into the plant, or portion of the plant, the modified HA protein comprises a modified proteolytic loop. Followed by incubating the plant or portion of the plant under conditions that permit the expression of the nucleic acids, thereby producing the VLP. The modified proteolytic loop may comprise one or more protease cleavage sites exhibiting reduced or abolished cleavage by a protease. The nucleotide sequence encoding the HA may be selected from the group consisting of B HA, C, H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15, and H16. Also described is a virus like particle (VLP) produced by the method, and plants expressing the VLP. The virus like particle (VLP) may comprise plant-specific N-glycans, or modified N- glycans.
公开/授权文献:
- CN105247059B 植物中流感样病毒颗粒的产生 公开/授权日：2021-07-06