基本信息:
- 专利标题： 一种用于扩增灰葡萄孢菌四个基因的方法及其多重PCR引物组
- 申请号：CN201510593450.0 申请日：2015-09-17
- 公开(公告)号：CN105154432B 公开(公告)日：2018-04-06
- 发明人： 马雪梅 , 张鑫 , 吕宝北 , 赵鹏翔 , 谢飞
- 申请人： 北京工业大学
- 申请人地址： 北京市朝阳区平乐园100号
- 专利权人： 北京工业大学
- 当前专利权人： 北京工业大学
- 当前专利权人地址： 北京市朝阳区平乐园100号
- 代理机构： 北京思海天达知识产权代理有限公司
- 代理人： 刘萍
- 主分类号： C12N15/10
- IPC分类号： C12N15/10 ; C12N15/11 ; C12Q1/686 ; C12Q1/04
The invention provides a method for amplifying four genes of Botrytis cinerea and a multiplex PCR (polymerase chain reaction) primer set thereof, relating to the field of molecular biology. On the basis of drug-resistant mutant sites of known Botrytis cinerea p-benzimidazole, boscalid, iprodione, fenhexamid and many other bactericides, a PCR primer set for simultaneously amplifying four gene segments beta-tub, BsOS1, erg27 and SdhB is established. The extracted Botrytis cinerea genome can be directly used as a template and subjected to amplification by using the designed primer set to obtain the enriched four gene segments. The multiplex PCR process is used for the first time to simultaneously amplify the four drug-resistant mutant genes of Botrytis cinerea, thereby greatly reducing the workload for drug-resistant site detection in the later period. By optimizing the PCR reaction system, the four gene segments are amplified in one PCR process.
公开/授权文献:
- CN105154432A 一种用于扩增灰葡萄孢菌四个基因的方法及其多重PCR引物组 公开/授权日：2015-12-16