基本信息:
- 专利标题： 一种肺癌miRNA检测试剂盒及其miRNA的应用
- 专利标题（英）：miRNA detection kit for lung cancer and application of miRNA
- 申请号：CN201510090767.2 申请日：2015-02-28
- 公开(公告)号：CN104593520A 公开(公告)日：2015-05-06
- 发明人： 赵新泰 , 王明 , 唐娟娟
- 申请人： 上海赛安生物医药科技有限公司
- 申请人地址： 上海市宝山区沪太路1888号文盛楼1楼
- 专利权人： 上海赛安生物医药科技有限公司
- 当前专利权人： 和卓生物科技(上海)有限公司
- 当前专利权人地址： 上海市宝山区沪太路1888号文盛楼1楼
- 代理机构： 上海海颂知识产权代理事务所
- 代理人： 任益
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
The invention relates to a combination of miR-21, miR-24, miR-125b and miR-126 used as a lung cancer molecular marker, relates to application of the combination of miR-21, miR-24, miR-125b, miR-126 and miR-39 to the preparation of miRNA detection kit for lung cancers, and relates to a miRNA detection kit for the lung cancer. An RCR reaction system comprises positive primers and a reverse universal primer aiming at miR-21, miR-24, miR-125b and miR-126; an internal reference system comprises an internal reference miRNA-39 and a positive primer aiming at miR-39; a nucleotide sequence of the positive primer aiming at r miR-21 is shown in SEQ ID No.1; a nucleotide sequence of the positive primer aiming at miR-24 is shown in SEQ ID No.2; a nucleotide sequence of the positive primer aiming at miR-125b is shown in SEQ ID No.3; a nucleotide sequence of the positive primer aiming at miR-126 is shown in SEQ ID No.4; a nucleotide sequence of the positive primer aiming at miR-39 is shown in SEQ ID No.5; a nucleotide sequence of the reverse universal primer is shown in SEQ ID No.6. The miRNA detection kit for lung cancer is high in detection speed, convenient and high in correction rate and can assist in early diagnosis of lung cancer.
公开/授权文献:
- CN104593520B 一种肺癌miRNA检测试剂盒及其miRNA的应用 公开/授权日：2016-10-05