The invention discloses an intermediate-infrared hollow optical fiber ATR coupling probe and belongs to the technical fields of biomedical photonics and molecular spectrometric measurement and devices. The intermediate-infrared hollow optical fiber ATR coupling probe comprises an ATR crystal probe (1), an incident optical fiber (2-1), an emergent optical fiber (2-2), a handle cover (3), a dual-optical-fiber protective layer (4), a single-optical-fiber protective layer (6), a connecting sleeve (5), an incident optical fiber jumper (7-1) and an emergent optical fiber jumper (7-2). With the adoption of a method for coupling an intermediate-infrared hollow optical fiber and a ZnSe crystal probe, the probe is flexible and convenient due to reasonable design, small-area single-point detection and in-situ measurement can be realized, spectral deformation caused by factors such as light scattering is avoided, the infrared spectroscopy quality is improved, the probe is suitable for in-vivo detection or living body detection and has wide application prospects in the field of biomedicines, and the application range of the ATR spectral measurement technology in the field of biomedical engineering is widened.