The invention discloses a gas and liquid distribution plate of an up-flow reactor and an inter-segment hydrogen supplementation device thereof. Before flowing into a catalyst bed, gas and liquid flow along independent channels and do not make contact with each other. In the operation process, the liquid phase enters an end enclosure of the reactor from a feed pipe on the bottom of the rector, is uniformly distributed through a porous plate, penetrates through a gas distribution chamber along a plurality of parallel liquid rising pipes to flow upwards and flows out through small holes in the side surfaces of the tops of the liquid rising pipes; gas enters the gas distribution chamber from an inlet pipe on the side surface of the reactor and flows out from small holes in the side surfaces of gas rising pipes arranged on the top of the gas distribution chamber. The liquid is limited to flow in the liquid rising pipes, the gas distribution chamber is only filled with the gas and is not disturbed by the liquid, and therefore the situation that the gas distribution chamber is polluted by the liquid and needs to be cleaned frequently is avoided. The gas and the liquid are uniformly mixed at the top of the distribution plate and then enter the catalyst bed. The liquid phase continues flowing upwards, consumed hydrogen is supplemented through the inter-segment hydrogen supplementation device, and therefore a reaction can go on.