The invention discloses a method for screening glyphosate resisting lolium multiflorum plants with shorter consumed time. The method comprises the steps of adopting mature lolium multiflorum seeds as explants, cleaning and sterilizing the mature lolium multiflorum seeds, then, inoculating to a callus induction culture medium, culturing for 18-22 days so as to obtain a callus, then, transferring the callus into a first callus subculture medium, culturing for 18-22 days, then, transferring to a second callus subculture medium containing glyphosate, culturing for 18-22 days, then, carrying out glyphosate resisting callus screening, then, transferring screened glyphosate resisting calluses into a callus differentiation culture medium, culturing for 18-22 days, transferring survival seedlings into a rooting culture medium when the seedlings grow to 2-3cm, culturing for 20-30 days, and finally, transplanting resistant seedlings. The plants with glyphosate resistance can be rapidly obtained by the method, and the required time is relatively short and is just about one hundred days; the method is suitable for being popularized and applied to the field of biotechnologies.