The invention discloses an inspection and calibration assembly for an osmometer, and aims to solve the problems of large size, high price and inconvenience in carrying in the conventional inspection and calibration assembly for osmometers. The inspection and calibration assembly comprises a calibration pipe barrel for accommodating the osmometer, an osmometer cable arranged at one end of the calibration pipe barrel, and a pressure meter inspector arranged at the other end of the calibration pipe barrel through a hollow connecting piece, wherein a sealing structure is arranged at one end, which is connected with the calibration pipe barrel, of the mosmometer cable; an enclosed detection space is formed among the calibration pipe barrel, the sealing joint of the osmometer cable and the pressure meter inspector. The inspection and calibration assembly is suitable for inspecting and calibrating under the specific condition of a small number of engineering safety monitoring osmometers, is simple in structure, convenient and rapid for installation and assembly, convenient to carry, low in cost and good in effect, and has no influence on the measuring accuracy.