基本信息:
- 专利标题： 基于适配体的磁纳米荧光传感器检测抗生素多残留的方法
- 专利标题（英）：Method for detecting multi-residues of antibiotics by magnetic nano fluorescence sensor based on aptamer
- 申请号：CN201410111739.X 申请日：2014-03-25
- 公开(公告)号：CN103852460A 公开(公告)日：2014-06-11
- 发明人： 卢春霞 , 唐宗贵 , 罗小玲 , 刘长彬 , 王远 , 贺玉凤 , 王国红 , 罗志根 , 刘丽 , 刘成江
- 申请人： 新疆农垦科学院
- 申请人地址： 新疆维吾尔自治区石河子市乌伊公路221号
- 专利权人： 新疆农垦科学院
- 当前专利权人： 新疆农垦科学院
- 当前专利权人地址： 新疆维吾尔自治区石河子市乌伊公路221号
- 主分类号： G01N21/64
- IPC分类号： G01N21/64
The invention relates to a method for detecting multi-residues of antibiotics by a magnetic nano fluorescence sensor based on an aptamer, belonging to the field of food safety analysis. According to the method, terramycin and kanamycin aptamers are simultaneously modified on the surface of a nano magnetic micro-sphere; complementary chains of fluorescence radical marks are respectively combined by base complementation; when an object to be detected exists, the aptamer is combined with the object to be detected to cause complementary chain dissociation to change fluorescence intensity, so that the aim of quantitatively detecting terramycin and kanamycin is realized. The method utilizes a magnetic nano material to rapidly separate the target to be detected in a sample so that the detection time is shortened, the detection cost is reduced, the detection sensitivity is improved, the operation is simple and the aim of analyzing the multi-residues is realized.
公开/授权文献:
- CN103852460B 基于适配体的磁纳米荧光传感器检测抗生素多残留的方法 公开/授权日：2016-03-02
IPC结构图谱:
| G | 物理 |
| --G01 | 测量；测试 |
| ----G01N | 借助于测定材料的化学或物理性质来测试或分析材料 |
| ------G01N21/00 | 利用光学手段，即利用红外光、可见光或紫外光来测试或分析材料 |
| --------G01N21/01 | .便于进行光学测试的装置或仪器 |
| ----------G01N21/63 | ..光学激发的 |
| ------------G01N21/64 | ...荧光；磷光 |