基本信息:
- 专利标题： 低岩藻糖细胞系及其应用
- 申请号：CN201280021537.5 申请日：2012-03-05
- 公开(公告)号：CN103597073B 公开(公告)日：2019-06-07
- 发明人： D·黑尔曼 , M·巴尔-西蒙 , M·托耶斯特-阿契杜夫
- 申请人： 默克雪兰诺有限公司
- 申请人地址： 瑞士科因辛斯
- 专利权人： 默克雪兰诺有限公司
- 当前专利权人： 默克雪兰诺有限公司
- 当前专利权人地址： 瑞士科因辛斯
- 代理机构： 上海专利商标事务所有限公司
- 代理人： 马彦
- 优先权： 211583 2011.03.06 IL
- 国际申请： PCT/IL2012/000109 2012.03.05
- 国际公布： WO2012/120500 EN 2012.09.13
- 进入国家日期： 2013-11-01
- 主分类号： C12N15/10
- IPC分类号： C12N15/10 ; C12N9/10 ; C12N15/01
A method of selecting cells having zero fucose level useful as host cells for expressing recombinant proteins is disclosed. The method comprises: (d) introducing genetic mutations into a population of CHO cells by contacting the cells with a methotrexate (MTX), (e) contacting the population of CHO cells comprising mutated cells with a non-toxic fucose binding agent for an amount of time that allows binding of the fucose binding agent to a fucose moiety on a cell membrane of the population of cells, wherein the amount of time does not allow killing of the cells; and (f) depleting from the population of cells comprising mutated cells, a subpopulation of cells which bind the fucose binding agent, thereby selecting cells useful as host cells for expressing recombinant proteins, the selected cells having zero fucose content. There are also disclosed cells and cell lines useful as host cells for expressing recombinant proteins.
公开/授权文献:
- CN103597073A 低岩藻糖细胞系及其应用 公开/授权日：2014-02-19