The invention provides Desmodium styracifolium total flavonoids. In the Desmodium styracifolium total flavonoids, the content of flavonoids (calculated as rutin) is 50-60 % (w/w), the total content of quercetin and kaempferol is 4-5 % (w/w). The invention further provides a method for extracting total flavonoids and total polysaccharides at the same time from Desmodium styracifolium. The invention further provides the use of Desmodium styracifolium total flavonoids and total polysaccharides. By the investigation of extraction and purification methods, the invention successfully extracts and separate Desmodium styracifolium total flavonoids and total polysaccharides at the same time from one and the same batch of pharmaceutical raw materials, so as to effectively utilize the pharmaceutical raw material resources and save the production cost, being more suitable for industrial production. Meanwhile, the invention further discloses that Desmodium styracifolium total flavonoids and total polysaccharides have good diuretic, calculus-removing and choleretic effects, which provides a guide for clinical medication.