基本信息:
- 专利标题： SIRT6的调节物及其筛选分析
- 专利标题（英）：Modulators for SIRT6 and assays for screening same
- 申请号：CN201180068116.3 申请日：2011-12-21
- 公开(公告)号：CN103403553A 公开(公告)日：2013-11-20
- 发明人： 林合宁
- 申请人： 康奈尔大学
- 申请人地址： 美国纽约州
- 专利权人： 康奈尔大学
- 当前专利权人： 康奈尔大学
- 当前专利权人地址： 美国纽约州
- 代理机构： 北京万慧达知识产权代理有限公司
- 代理人： 白华胜; 段晓玲
- 优先权： 61/426,231 2010.12.22 US
- 国际申请： PCT/US2011/066480 2011.12.21
- 国际公布： WO2012/088268 EN 2012.06.28
- 进入国家日期： 2013-08-21
- 主分类号： G01N33/68
- IPC分类号： G01N33/68 ; C07C225/06 ; C12Q1/34 ; A61K31/12 ; A61K31/131 ; G01N33/15
Method for identifying a modulator of Sirt6, PfSir2a, or Sirt7 deacylase activity, wherein a fatty-acylated substrate containing an acyl-lysine moiety and an indicator moiety is contacted with Sirt6, PfSir2a, or Sirt7 in the presence of a candidate compound under conditions for Sirt6, PfSir2a, or Sirt7 to deacylate the substrate, wherein the acyl is a hydrophobic fatty acyl group containing a hydrocarbon group having at least three carbon atoms connected by carbon-carbon bonds; contacting the deacylated substrate with a cleavage agent that cleaves the linkage between the lysine and indicator moiety to generate a detectable signal; and correlating a quantified Sirt6, PfSir2a, or Sirt7 deacylase activity therefrom. Modulating compounds of Sirt6, PfSir2a, or Sirt7 deacylase activity are also described, as are pharmaceutical compositions thereof, methods of treatment by administration of the modulating compounds, and kits for practicing the method.
公开/授权文献:
- CN103403553B SIRT6的调节物及其筛选分析 公开/授权日：2016-03-09