The invention discloses a decoding card and a card reader and an application system which are matched with the same. The decoding card comprises paper, a film, a metal slice and a card base made of at least one substance in an organic glass slice. A conductive coating is coated on the surface of the card base and provided with a plurality of conductive logic expression areas, each conductive logic expression area is provided with an independent conductive performance, and the conductive performances of the conductive logic expression areas on the conductive coating form a unique conductive logic data code used for identifying the decoding card and distinguishing the decoding card from other decoding cards. The card reader matched with the decoding card is further provided. By means of the decoding card, the card reader and the application system, data safety can be improved, and a plurality of decoding cards can be specially made and are independent and not repeated, so that various different roles can be provided in the application, function selectivity of the application can be improved, and game entertainment is improved as for games.