基本信息:
- 专利标题： 一种高富硒钝顶螺旋藻的培养及其检测方法
- 专利标题（英）：Method for culturing and detecting high selenium-enriched spirulina platensis
- 申请号：CN201210275399.5 申请日：2012-08-03
- 公开(公告)号：CN102876579A 公开(公告)日：2013-01-16
- 发明人： 黄蓓 , 王鑫焱 , 沈敬连 , 武子涵 , 刘亚华
- 申请人： 安徽大学 , 亳州市贡药饮片厂
- 申请人地址： 安徽省合肥市肥西路3号安徽大学龙河校区
- 专利权人： 安徽大学,亳州市贡药饮片厂
- 当前专利权人： 安徽大学,亳州市贡药饮片厂
- 当前专利权人地址： 安徽省合肥市肥西路3号安徽大学龙河校区
- 代理机构： 安徽合肥华信知识产权代理有限公司
- 代理人： 余成俊
- 主分类号： C12N1/12
- IPC分类号： C12N1/12 ; G01N21/64 ; C12R1/89
The invention discloses a method for culturing and detecting high selenium-enriched spirulina platensis. The culture method comprises the following steps of: illuminating and culturing at temperature of between 20 and 25 DEG C for 24 hours every day under the conditions that the pH value is 8 to 10, the illumination intensity is 2,000 to 2,500 lx; culturing in a triangular culture flask by using a Zarrouk culture solution under the condition that the inoculum density OD560 is 0.25 to 0.35; adding Na2SeO3 sequentially until the total selenium amount is 270 to 300 mg/L; and when the OD 560 is 1.2 to 1.5, collecting spirulina platensis, and freeze-drying to prepare the high selenium-enriched spirulina platensis. According to the method, the culture condition is easy to control; and the spirulina platensis is high in reproduction speed, short in growth period and low in cost, the total selenium content is 2.0 to 2.5 mg/g, the content of organic selenium is 68 to 80 percent, and the content of essential amino acid and immunity are improved greatly.
公开/授权文献:
- CN102876579B 一种高富硒钝顶螺旋藻的培养及其检测方法 公开/授权日：2014-06-11