The invention discloses a measuring device of the effective diffusion coefficient of a weak adsorption gas in a bulk nano-porous material under low pressure and application thereof. The measuring device comprises a vacuum container system, a data acquisition system, a gas supply bottle, and the like, wherein the vacuum container system comprises a sample pool, a gas supply pool, a reference pool, a calibration pool, a buffer tank and a vacuum pump, the sample pool passes through a valve through a pipeline to be connected with the gas supply pool, the gas supply pool is provided with a pipeline which passes through the valve to be connected with the reference pool, the gas supply pool is also provided with a pipeline which is communicated with a double tee and then passes through the valve to be respectively connected with the calibration pool, the buffer tank and the vacuum pump, and the buffer tank passes through the valve through the pipeline to be connected with the gas supply bottle; the data acquisition system comprises a data acquisition unit and a computer, wherein the computer is connected with the data acquisition unit, and the data acquisition unit acquires data and then transmits to the computer connected with the data acquisition unit. The measuring device is simple in structure and suitable for measuring the effective diffusion coefficient of the weak adsorption gas in the bulk nano-porous material under the low pressure.