基本信息:
- 专利标题： 一种中温耐碱性甘露聚糖酶Man5XH9及其基因和应用
- 专利标题（英）：Medium-temperature alkali-resistant mannase Man5XH9 and gene and application thereof
- 申请号：CN201110334811.1 申请日：2011-10-28
- 公开(公告)号：CN102329785A 公开(公告)日：2012-01-25
- 发明人： 詹志春 , 张菁
- 申请人： 武汉新华扬生物股份有限公司
- 申请人地址： 湖北省武汉市东湖新技术开发区凌家山南路5号
- 专利权人： 武汉新华扬生物股份有限公司
- 当前专利权人： 武汉新华扬生物股份有限公司
- 当前专利权人地址： 湖北省武汉市东湖新技术开发区凌家山南路5号
- 代理机构： 北京法思腾知识产权代理有限公司
- 代理人： 高宇; 杨小蓉
- 生物保藏信息： CGMCC No. 5369 2011.10.21
- 主分类号： C12N9/42
- IPC分类号： C12N9/42 ; C12N15/56 ; C12N15/63 ; C12N1/15 ; C12N1/19 ; C12N1/21 ; C12N1/14 ; C12R1/80
The invention relates to the field of genetic engineering, in particular to medium-temperature alkali-resistant mannase Man5XH9 and a gene and application thereof. The invention provides the novel medium-temperature alkali-resistant mannase Man5XH9 which has an amino acid sequence shown as SEQ ID NO.1 or 2; and the invention also provides a gene for encoding the medium-temperature alkali-resistant mannase Man5XH9, a recombinant carrier and a recombinant strain which contain the gene, and application of the medium-temperature alkali-resistant mannase Man5XH9, wherein a nucleotide sequence of the gene is shown as SEQ ID NO. 4 or 5. The optimum pH value of the mannase Man5XH9 is 4.5, the mannase Man5XH9 has higher enzymatic activity at the pH value of between 4.0 and 7.0, the pH stability ishigh, and the mannase Man5XH9 can be applied to papermaking and textile industries.
公开/授权文献:
- CN102329785B 一种中温耐碱性甘露聚糖酶Man5XH9及其基因和应用 公开/授权日：2013-03-20