The invention discloses a method for extracting passion flower seed oil by utilizing an aqueous enzymatic method. At present, the extraction methods of the passion flower seed oil comprise a squeezing method, a biological grinding method, a solvent extraction method, a microwave extraction method and a supercritical extraction method, and the methods have various disadvantages, such as low extraction rate of oil, overlong extraction time and higher extraction cost. In the invention, the passion flower seed oil is extracted by utilizing the aqueous enzymatic method, and oil and non-oil ingredients can be separated by utilizing the affinity difference of the non-oil ingredients between oil and water and different specific weights of oil and water. The high-grade passion flower seed oil prepared from passion flower seeds by utilizing the method disclosed by the invention has the extraction rate up to more than 20 percent; and the obtain oleic acid has low cost, shallow color, no aflatoxin pollution, no solvent residue, shorter extraction time and lower extraction cost.