基本信息:
- 专利标题： 提高轮枝拟青霉GZ中虫草菌素产量的方法
- 专利标题（英）：Method for improving yield of cordycepin in Verticillium Paecilomyces GZ
- 申请号：CN201010243545.7 申请日：2010-08-03
- 公开(公告)号：CN101962665A 公开(公告)日：2011-02-02
- 发明人： 李祝 , 任秀秀 , 肖洋 , 陈青 , 周礼红 , 刘吴娟
- 申请人： 贵州大学
- 申请人地址： 贵州省贵阳市花溪区贵州大学(北区)科技处
- 专利权人： 贵州大学
- 当前专利权人： 贵州大学,贵州省产品质量监督检验院
- 当前专利权人地址： 贵州省贵阳市花溪区贵州大学(北区)科技处
- 代理机构： 贵阳东圣专利商标事务有限公司
- 代理人： 袁庆云
- 生物保藏信息： CCTCC NO: M 209302 2009.12.14
- 主分类号： C12P19/40
- IPC分类号： C12P19/40 ; C12R1/79
The invention discloses a method for improving yield of cordycepin in Verticillium Paecilomyces, comprising: GZ is inoculated on a PDA inclined plane culture medium; at the temperature of 26 DEG C, GZ is cultured for 5d and activated for 3 times; then, the obtained GZ is inoculated into a PDA liquid seed culture medium in a v/v ratio of 5%; at 140 rpm and the temperature of 26 DEG C, the mixture is cultured in a shake flask for 3d to obtain the liquid female seed; the liquid female seed, in a v/v ratio of 5%, is inoculated in a fermentation culture medium containing 2% of white granulated sugar, 1% of maltose, 2% of beef extract and 3% of yeast extract; meanwhile, cetane, in a v/v ratio of 2-8%, is added; and at 140 rpm and the temperature of 26 DEG C, the cordycepin is obtained by culturing the mixture in the shake flask for 7d.The method only needs stable and simple fermentation condition, is easy for culturing thallus, has good effect on improving cordycepin output and can realize commercial scale production.
公开/授权文献:
- CN101962665B 提高轮枝拟青霉GZ中虫草菌素产量的方法 公开/授权日：2012-12-26
IPC结构图谱:
| C | 化学；冶金 |
| --C12 | 生物化学；啤酒；烈性酒；果汁酒；醋；微生物学；酶学；突变或遗传工程 |
| ----C12P | 发酵或使用酶的方法合成目标化合物或组合物或从外消旋混合物中分离旋光异构体 |
| ------C12P19/00 | 含有糖残基的化合物的制备 |
| --------C12P19/26 | .含氮碳水化合物的制备 |
| ----------C12P19/28 | ..N-葡萄糖苷 |
| ------------C12P19/30 | ...核苷酸 |
| --------------C12P19/40 | ....含有在同一环上有两个氮原子的六元环的稠合环体系，如嘌呤核苷 |