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1. 发明申请

WO2023086967A1 METHOD OF ANALYSIS OF METHYLATED DNA-BINDING PROTEINS 审中-公开
公开(公告)号：WO2023086967A1
公开(公告)日：2023-05-19
申请号：PCT/US2022/079757
申请日：2022-11-11
申请人： GUARDANT HEALTH, INC.
发明人： OHSHIMA, Saiyou , STAPLETON, Kenneth Edmund , HITE, Dustin Howard , CHEN, Xiao-Bo , LIN, Chia-Hui , ANNUAR, Dania , PIERACCI, Jessica Michelle
IPC分类号： G01N33/53 , G01N33/68 , G01N33/543 , G01N33/566 , C12Q1/6804 , C12Q1/6827
摘要： Provided herein are methods of assaying a methylated DNA-binding protein comprising contacting methylated DNA-binding proteins with labeled oligonucleotides, obtaining sample partitions for oligonucleotides with different methylation states, and quantifying the labeled oligonucleotides in the partitions.

2. 发明申请

WO2022195089A1 METHODS FOR THE SELECTIVE ANALYSIS OF CELLS OR ORGANELLES 审中-公开
公开(公告)号：WO2022195089A1
公开(公告)日：2022-09-22
申请号：PCT/EP2022/057187
申请日：2022-03-18
申请人： PARIS SCIENCES ET LETTRES , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE , ECOLE SUPERIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS
发明人： RONDELEZ, Yannick , GRIFFITHS, Andrew , BLIVET-BAILLY, Gaël , IBANEZ, Pablo , BANERJEE, Satyam , GINES, Guillaume , MASURIER, Antoine
IPC分类号： C12Q1/6804 , C12N15/10 , C12Q1/682
摘要： The present invention relates to a method for the selective analysis of a subpopulation of cells or organelles based determining the level of at least one cell or organelle marker, via the implementation of an adapted DNA toolbox able to convert the presence of a cell or organelle marker into a signal. This method is particularly useful for selecting cells or organelles having a phenotype of interest, analyzing the proteins of said cells or organelles and/or sequencing the DNA and/or RNA of said cells. The present invention also relates to a kit for implementing the method of selective analysis of cells or organelles as defined above.

3. 发明申请

WO2022182672A1 SINGLE CELL GLYCAN PROFILING 审中-公开
公开(公告)号：WO2022182672A1
公开(公告)日：2022-09-01
申请号：PCT/US2022/017356
申请日：2022-02-22
申请人： 10X GENOMICS, INC.
发明人： PATTERSON, David Michael
IPC分类号： C12Q1/6804 , C12Q1/6806 , C12Q1/48
摘要： The present disclosure relates to methods, compositions, systems, and kits for detecting and analyzing the glycosylation of healthy and diseased cells and protein-specific glycosylation patterns using single-cell profiling methodologies.

4. 发明申请

WO2022175483A1 METHOD AND KITS FOR MULTIPLEXED FLUORESCENT MICROSCOPY 审中-公开
公开(公告)号：WO2022175483A1
公开(公告)日：2022-08-25
申请号：PCT/EP2022/054140
申请日：2022-02-18
申请人： MICROGRAPHIA BIO LIMITED
发明人： ADJALLEY, Sophie , THOMPSON, Christopher C.
IPC分类号： C12Q1/6804
摘要： Provided is a method for multiplexed fluorescence microscopy comprising contacting a fixed sample with a set of binding agent-T-oligonucleotide conjugates to allow the binding agents to bind to any binding partners present in the sample, wherein the set comprises a plurality of binding agents having different specificities and the sequence of the T-oligonucleotide is unique to the binding agent to which it is conjugated, contacting the sample and any bound binding agents resulting from step a with a FRET- oligonucleotide, illuminating the sample with a wavelength to cause excitation of the FRET- oligonucleotide's emitter molecule, and observing the fluorescent kinetic profile of the sample at the FRET-oligonucleotide emitter molecule's emission wavelength at one or more pixels over time, wherein the FRET-oligonucleotide can hybridise to multiple T-oligonucleotides in the set, to form multiple pairs, and wherein the dissociation and reassociation between each different pair generates a fluorescent kinetic profile that is unique within that set to that pair. Also provided are associated kits, sets of binding agent T-oligonucleotide conjugates and corresponding FRET-oligonucleotides, and methods of designing such sets.

5. 发明申请

WO2022164893A1 QUANTIFICATION OF CELLULAR PROTEINS USING BARCODED BINDING MOIETIES 审中-公开
公开(公告)号：WO2022164893A1
公开(公告)日：2022-08-04
申请号：PCT/US2022/013885
申请日：2022-01-26
申请人： CHAN ZUCKERBERG BIOHUB, INC. , THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
发明人： GREENLEAF, William J. , CHEN, Amy F.
IPC分类号： G01N33/53 , C07K14/195 , C07K14/47 , C12Q1/6804
摘要： The invention provides compositions and methods for quantifying cellular molecules of interest, e.g., intracellular proteins, using oligonucleotide-target binding moiety conjugates.

6. 发明申请

WO2022133107A8 METHODS FOR DIAGNOSING ALZHEIMER'S DISEASE AND OTHER NEUROLOGICAL DISORDERS 审中-公开
公开(公告)号：WO2022133107A8
公开(公告)日：2022-06-23
申请号：PCT/US2021/063843
申请日：2021-12-16
申请人： COGNEXT DIAGNOSTICS LLC , THE JOHNS HOPKINS UNIVERSITY
发明人： WORLEY, Paul, F. , XIAO, Meifang , XU, Desheng
IPC分类号： G01N33/68 , C07K16/18 , G01N33/566 , G01N33/53 , C12Q1/6804 , C12Q1/6851 , G01N2800/2814 , G01N2800/52 , G01N33/6896
摘要： Disclosed herein are methods for diagnosing a subject as having a neurological disorder, such as Alzheimer's disease, using proximity-based detection assays capable of detecting complexes containing two or more synaptic proteins, such as neuronal pentraxins, from a blood, cerebrospinal fluid, or other fluid sample of the subject. Also disclosed are methods of evaluating and monitoring subjects having or at risk of developing a neurological disorder using the aforementioned assays.

7. 发明申请

WO2022125755A1 METHOD AND COMPOSITION FOR MULTIPLEXED AND MULTIMODAL SINGLE CELL ANALYSIS 审中-公开
公开(公告)号：WO2022125755A1
公开(公告)日：2022-06-16
申请号：PCT/US2021/062575
申请日：2021-12-09
申请人： THERMO FISHER SCIENTIFIC INC. [US]/[US] , PHITONEX, INC. [US]/[US] , PINKIN, Nicholas
发明人： STADNISKY, Michael , LABODA, Craig , GODINEZ, Ivan
IPC分类号： C12Q1/6804
摘要： Provided herein are multimodal methods and compositions that combine sequence-tagged antibodies and fluorescent labels in a single reagent. Combined with optimal panel design, high-purity sorting of cells before sequencing has been demonstrated, and furthermore, truly quantitative information on the cell surface markers used for sorting.

8. 发明申请

WO2022109339A8 USE OF DEXTRAMER IN SINGLE CELL ANALYSIS 审中-公开
公开(公告)号：WO2022109339A8
公开(公告)日：2022-05-27
申请号：PCT/US2021/060197
申请日：2021-11-19
申请人： BECTON, DICKINSON AND COMPANY , IMMUDEX APS
发明人： SAKOFSKY, Cynthia , LAZARUK, Katherine , NAKAMOTO, Margaret , JENSEN, Devon Joseph , JACOBSEN, Kivin Dahl
IPC分类号： C12Q1/6804 , C12Q1/6806 , C12Q1/6869 , G01N33/50 , C12Q1/6874 , G01N2458/10 , G01N33/56972
摘要： Disclosed herein include systems, methods, compositions, and kits suitable for the use of dextramers in single cell analysis. In some embodiments, one or more primers allowing generation of separate libraries for proteins (e.g., antibodies) and for dextramers are used.

9. 发明申请

WO2022102791A1 新規抗原検出用リンカー及びそれを用いた抗原検出方法审中-公开
公开(公告)号：WO2022102791A1
公开(公告)日：2022-05-19
申请号：PCT/JP2021/042138
申请日：2021-11-16
申请人：国立大学法人埼玉大学 , 株式会社Ｅｐｓｉｌｏｎ　Ｍｏｌｅｃｕｌａｒ　Ｅｎｇｉｎｅｅｒｉｎｇ
发明人：根本　直人 , 東出　壮
IPC分类号： C12N15/11 , C12N15/115 , C12Q1/6804
摘要：本発明は、高感度かつ迅速な検出が可能な抗原の測定系を提供することを目的とする。具体的には、主鎖と側鎖とを有する抗原検出用新規リンカーであって、主鎖と側鎖とを有する抗原検出用新規リンカーであって、（１）前記主鎖は、側鎖結合部位と、第１の架橋用化合物を含むmRNA結合領域と、第２の架橋用化合物を含む蛍光検出用DNA結合領域と、前記蛍光検出用DNA結合領域の５'側に位置する固相切断部位と、主鎖の５'末端に位置する固相結合部位とを備え；（２）前記側鎖は、主鎖結合部位と、ペプチド提示部位と、を備える；抗原検出用新規リンカー、及びこのリンカーを用いた抗原検出方法を提供する。　[選択図]　図４

10. 发明申请

WO2022076914A1 METHODS AND COMPOSITIONS FOR PROFILING IMMUNE REPERTOIRE 审中-公开
公开(公告)号：WO2022076914A1
公开(公告)日：2022-04-14
申请号：PCT/US2021/054307
申请日：2021-10-08
申请人： 10X GENOMICS, INC.
发明人： MCDONNELL, James Wyatt
IPC分类号： C12N15/10 , C12Q1/6804
摘要： The present disclosure relates in some aspects to methods and compositions for analyzing a biomolecule, such as an antibody, and/or analyzing a repertoire comprising biomolecules, such as the repertoire of antibody binding specificities in a sample comprising one or more antibodies. Exemplary samples include serum and plasma, as well as monoclonal antibody compositions.

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